Homologous and heterologous cell absorption of cytotoxicity toward homologous cells

Both negative (1-5) and positive (6-9) evidence may be found in the literature for the occurrence of tumor-specific cytotoxic antibody in heterologous antisera to mammalian tumor cells. The negative evidence appears to have been due to the intervention of species-specific antibody. In a further examination of this question, we have studied the cytotoxicity and absorption reactions of rabbit antisera to cultured human cell lines and strains. Our experiments centered around the use of cell cultures of three types of tumors: sarcoma (SV40 virus-transformed human embryonic lung), carcinoma (HeLa), and lymphoma (EB2).